#COMPRESSED AURICULA FREE#

At present, most researches have focused on the extraction and bioactivity of the polysaccharides from A.В auricular (Bao et al., 2017 Qiu, Zhang, Wang, Zhang, Wang, Liu, & Regenstein, 2016 Xu et al., 2018).

Growing concerns over the huge amount of substrate wastes of A.В auricula to the squander of resources and the contamination of environmental comes with the boom of A.В auricula industry. Modern pharmacological studies have shown that A.В auricula have many biological activities such as antioxidant (Xu et al., 2018), improve immunity (Nguyen, Wang, et al., 2012), and antitumor (Ma et al., 2018).

#COMPRESSED AURICULA FREE#

Melanin has many biological activities, such as eliminate free radical (Kim et al., 2017), antitumor (Shi et al., 2018), and anti-radiation (Ye et al., 2014).ДЄuricularia auricula is a popular edible mushroom in East Asia, especially in China, Japan, and Korea (Nguyen, Chen, et al., 2012 SД™kara, Kalisz, Grabowska, Kalisz, Grabowska, & Siwulski, 2015). Melanin is a kind of irregular pigment that composed of phenolic or indolic monomers, existing in animals, plants, and microorganisms (Huang et al., 2018). In addition to create the ideal color, natural pigments can also be used as functional ingredients (Li, Zhang, & Liu, 2013). At present, the safety of synthetic pigments has been paid more and more attention, and the application of natural pigments has aroused people's interest. Cell morphology observation confirmed that the melanin ameliorated the morphological changes of cells induced by oxidative stress. Waste residue melanin significantly inhibited the cell death caused by H 2O 2, and the cell viability was restored to 98.09В В±В 5.97% when the melanin concentration was 1.6В mg/ml. There was no significant difference between A.В auricula melanin control group and waste residue melanin on ABTS, DPPH, and hydroxyl radical scavenging activity. Melanin had a good solubility in alkali solution and exhibited a certain stability to thermal. The results indicated that the ultrasonic-assisted extraction process could be used to extract the melanin from A.В auricula waste residue. Furthermore, the chemical antioxidant activity of waste residue melanin and its protective effect on cell oxidative injury induced by H 2O 2 were investigated. In order to promote the comprehensive utilization of the Auricularia auricula waste residue, the extraction process and the physicochemical properties of melanin from A.В auricula waste residue were studied.

Compressed auricula